Synthesis and Evaluation of the Cytotoxic and Anti-Proliferative Properties of Dox-ZnO Quantum Dots Loaded Chitosan Nanoparticles against MCF-7 and SKBR-3 Human Breast Cancer Cells

Synthesis and Evaluation of the Cytotoxic and Anti-Proliferative Properties of Dox-ZnO Quantum Dots Loaded Chitosan Nanoparticles against MCF-7 and SKBR-3 Human Breast Cancer Cells



Srikanth Jagadeesan, Roshini A, Kim Kyung Hwan, Yang-Hoi Doh, Yoon-Kyu Lim, Kyung Hyun Choi



Doxorubicin (Dox) is a potent chemotherapeutic agent used in the
treatment of cancer. In the present study, pH responsive chitosan
polymer coated Dox nanoparticle (Composite) was developed to investigate
targeted drug delivery against breast cancer. The anticancer drug
DOX-ZnO QDs was loaded to the chitosan nanoparticles. The synthesized
free and drug loaded nanoparticle were analyzed using Fourier
transmission electron microscopy (FTIR) and UV-Visible
spectroscopy(UV-Vis). The particle size was measured using Transmission
Electron Microscopy (TEM). Further, the composite was evaluated for its
anticancer effects. Drug release analysis showed significantly larger
amount of drug released in acidic pH of 5.0 compared to pH 7.4. The
composite was significantly more cytotoxic to the breast cancer cells
MCF-7 and SKBR-3. The composite was however, less toxic to HEK-293 human
embryonic kidney cells confirming minimum side effects on normal cells
andcytotoxic to tumor cells. DAPI staining showed nuclear degradation in
composite treated breast cancer cells. The cellular uptake of the
composite was analyzed by confocal microscopy. The composite induced a
G0/G1 phase arrest in breast cancer cells and the number of colonies
formed by the composite treated breast cancer cells formed less number
of colonies compared to free NP. Our results showed that our composite
could serve as a promising therapeutic approach to improve clinical
outcomes against various malignancies. 



10.22161/ijeab/2.3.10



http://ijeab.com/upload_document/issue_files/10%20IJEAB-MAY-2017-16-Synthesis%20and%20Evaluation%20of%20the%20Cytotoxic%20and%20Anti-Proliferative%20Properties.pdf



http://ijeab.com/submit-paper/

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