Response of Running Shoot tips of Strawberry (Fragaria x ananasa) for in vitro Propagation in Kurdistan Region of Iraq

Response of Running Shoot tips of Strawberry (Fragaria x ananasa) for in vitro Propagation in Kurdistan Region of Iraq



Gharbia H. Danial , Diaa A. Ibrahim , Mobasher S. Omer



Strawberry (Fragaria x ananasa) is grown for its excellent food and
nutritional value in many parts of the world. The main objective of this
study was to optimize an efficient, reliable and economical protocol
for In vitro propagation of strawberry plants. The effect of BAP
(6-Benzyl amino purine) alone or combined with (Gibberellic acid,
Kinetin, IBA) and the effects of TDZ (Thidiazuron) on shoot
proliferation of strawberry (Fragaria x ananasa) has been evaluated. It
has been noticed that 2.0 mgl-1 BAP combination with 0.25 mgl-1 IBA able
to generate the maximum number of shoots / explants (6.375) with high
shoot length ( 0.675 cm) and high leaves number /explants (19.0). On
the other hand, presence of 1.5 mgl-1 of TDZ in the nutrient medium
increased the shoot number/explants to (7.335) as compared to BAP
treatments. The proliferated shoots were subjected to full strength MS
media supplemented with 0.75 mgl-1 IAA give the optimal ratio of
rooting. Survived rooted plantlets were acclimatized gradually and
successfully transferred under greenhouse conditions for hardening. 



10.22161/ijeab/1.2.11



http://ijeab.com/upload_document/issue_files/14%20IJEAB-JUL-2016-32-Response%20of%20Running%20Shoot%20tips%20of%20Strawberry%20(Fragaria%20x%20ananasa)%20for%20in%20vitro%20Propagation%20in%20Kurdistan%20Region%20of%20Iraq.pdf



ijeab.com/submit-paper/

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