Pathogenic and Molecular Characterization of Fusarium oxysporum f.sp. ciceri Causing Chickpea Wilt through ISSR Markers

Pathogenic and Molecular Characterization of Fusarium oxysporum f.sp. ciceri Causing Chickpea Wilt through ISSR Markers



Pravin D.Gajbhe, Madhuri Katkar, S.S.Mane, P.V.Jadhav



In the present investigation the pathogenic and
genetic variability was assayed, amongst the seven isolates of Fusarium
oxysporum f.sp. ciceri (Foc) collected from different agro-climatic
zones of Maharashtra State, India. The isolates of Fusarium oxysporum
f.sp.ciceri were confirmed by SCAR marker which yielded 1.5 KD band.
The pathogenicity of each isolate was confirmed using the wilt
susceptible chickpea genotype JG-62. On the basis of pathogenic ability
the isolates were grouped as highly pathogenic (FOC-2, FOC-5, FOC-6),
strongly pathogenic (FOC-1,FOC-3) and moderately pathogenic
(FOC-4,FOC-7). Eight Inter Simple Sequence Repeats primers (ISSRs) were
used to determine the genetic variability in seven isolates Fusarium
oxysporum f.sp. ciceri. The seven primers produced 80 scorable bands.
Off 80 bands, 73 bands were polymorphic and average level of
polymorphism was 91.25 per cent. In UPGMA analysis, Foc-1 (Wardha) was
found to have higher value of similarity coefficient (0.8375) whereas
Foc-2 (Lonar) was found to have lower value of similarity coefficient
(0.4625). The isolates of Fusarium oxysporum f.sp.ciceri were grouped
into two major clusters. First group, cluster-A includes isolates
belonging to Wardha, Washim and Lonar. Second group, cluster-B includes
Nashik, Ahmednagar, Rahuri and Pune. It shows that Foc-1 (Wardha) have
higher value of similarity coefficient with Foc-3 (Washim) whereas Foc-2
(Lonar) have lower value of similarity coefficient with Foc-5
(Ahmednagar).The similarity matrix indicated that seven isolates of
Fusarium oxysporum f.sp.ciceri exhibited in between 46-84 per cent
similarity coefficient. 



10.22161/ijeab/1.2.1



http://ijeab.com/upload_document/issue_files/21%20IJEAB-AUG-2016-14-Pathogenic%20and%20Molecular%20Characterization%20of%20Fusarium%20oxysporum%20f.sp.%20ciceri%20Causing%20Chickpea%20Wilt%20through%20ISSR%20Markers.pdf



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