Bacterial and Fungal Contamination of Air conditioners filters and Carpets

Bacterial and Fungal Contamination of Air conditioners filters and Carpets



Haitam Khalaf Almoffarreh, Faris Mohammed Alsaleh, Mansur Sultan Alruwaili



Background:
Objective: To study the level of colonization by bacteria, and
fungi/molds in air conditioners and carpets used in homes,
offices,university classrooms, prayer room and laboratories.
Methods: A total of 25 different settled dust samples from air
conditioners, and carpet vacuum cleaners were investigated in this
study. One gram of each sample was emulsified into 9.0mL sterile normal
saline. 10-fold dilutions were made in normal saline and 100 µL of each
suspension was inoculated onto 2 plates of SabouraudDextrose
Agarincubated at 25 °C for 48-72hrs, while 50 µLwas inoculated onto
MacConkey and Blood agar plates which were incubated at 37 °C for 48hrs.

Results: of the settled dust samples examined, 72 % were found to harbor
molds, bacteria and yeasts. Of the total bacteria isolated, Bacillus
spp. comprised 60.3%, S.epidermidis (20.7%), S. aureus (17.2%), Gram
negative bacilli (1.7%). On the other hand, Aspergillusspp. made up
26.9%, of the total mold isolates, followed byPenicillium spp. (20.9%),
Yeast (13.4%), Rhizopus spp. (7.5%), Zygomycetes spp. (3%),
Aspergillusniger (3%), Lichtheimiacorymbifera (3%), Microsporum spp.
(1.5%) and Unidentified mold in 20.8%. The mean bacterial concentration
found was 1.33 x 105Cfu/g of dust.
Conclusion: The presence of potentially pathogenic bacteria and molds in
the dust samples of the air conditioner filters, and home carpets
indicated that our environment is full of microorganism that may affect
people’s health and expose them to different diseases, this mandates
regular cleaning and safe removal of dust from A/C filters and carpets. 



10.22161/ijeab/1.3.14



http://ijeab.com/upload_document/issue_files/14%20IJEAB-SEP-2016-16-Bacterial%20and%20Fungal%20Contamination%20of%20Air%20conditioners%20filters%20and%20Carpets.pdf



http://ijeab.com/submit-paper/

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