Pathological, Bio-chemical and Molecular diversity amongst the isolates of Xanthomonas axonopodis pv. citri causing Citrus canker in acid lime from different agro-climatic region of India

Pathological, Bio-chemical and Molecular diversity amongst the isolates of Xanthomonas axonopodis pv. citri causing Citrus canker in acid lime from different agro-climatic region of India



Madhuri Katkar, K.S.Raghuwanshi, V. P. Chimote, S.G.Borkar



In present investigation isolates of Xanthomonas axonopodis pv. citri
(Xac)causing citrus canker were collected from fourteen agro climatic
regions of India. The pathogenic variability of Xac was studied on four
different varieties of acid lime viz. Sai sarbati, Phule sarbati,
Pramalini and PKM-1 by using detached leaf assay. The isolates showed
varied reaction in the symptoms development. The isolates viz. Xac- III,
Xac- V, Xac- VII, Xac- XI, Xac- XIII and Xac- XIV found highly virulent
and showed of typical symptoms at the point of inoculation within 7 to 9
days. The isolates Xac- I, Xac- II and Xac- IV were found less
virulent, developed symptoms after 13 to 16 days of inoculation. The
isolates Xac- I and Xac- II failed to develop symptom on variety PKM-1.
Further all 15 selected isolates were subjected to biochemical
characterization; all isolates were found rod shaped, gram –ve, with
colony colour ranging from pale yellow to dark yellow. The isolate were
positive for Catalase, KOH and H2S production, hydrolyse starch and
gelatin liquefaction. All isolates produce acid from Trahalose. Whereas
all isolates fails to produce Indol. The Random Amplified Polymorphic
DNA (RAPD) was used to study the variation amongst the 15 isolates of
Xac. A total of 27 RAPD primers were screened. Off which 19 primers
showed amplification and produced scorable bands with high degree of
polymorphism. A total 220 amplicons were obtained of which 218 amplicons
were polymorphic with 99.52% level of polymorphism. The banding profile
varied from minimum 5 band types (OPB- 1) to maximum 21 band type (REP)
indicating the high molecular variability amongst all the fifteen
isolates of Xac.The similarity coefficient ranged from 0.27 to 0.68. The
maximum genetic similarity was found amongst the isolate from Uttar
Pradesh (Xac- V) and Shriganganagar (Xac- XIV) i.e. 0.68 



10.22161/ijeab/1.2.25



http://ijeab.com/upload_document/issue_files/30%20IJEAB-AUG-2016-20-Pathological,%20Bio-chemical%20and%20Molecular%20diversity%20amongst%20the%20isolates%20of%20Xanthomonas%20axonopodis.pdf



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