Microwave-Assisted Alkali Delignification Coupled with Non-Ionic Surfactant Effect on the Fermentable Sugar Yield from Agricultural Residues of Cassava

Microwave-Assisted Alkali Delignification Coupled with Non-Ionic Surfactant Effect on the Fermentable Sugar Yield from Agricultural Residues of Cassava



Pooja N. S, Padmaja G



Cassava stem, leaves and peel are agricultural residues generated as
waste biomass during the cultivation and processing of cassava. The
potential of these biomasses as feedstock for ethanol production depends
on the effective deconstruction via pretreatment and saccharification.
The effect of alkaline hydrogen peroxide (AHP) treatment on microwave
(MW)-irradiated or steam-exposed aqueous slurry was compared with
MW-irradiation (300 W) of alkali slurry in delignifying the biomass and
degrading the polysaccharides. Cellulose was degraded to a higher extent
than hemicellulose in the AHP treatments. The steam-exposed and AHP
pretreated residues on saccharification with Cellic (Cellulase complex)
alone or Cellic along with Tween 20 resulted in high conversion of
carbohydrate to reducing sugars (RS) in leaves (64-70%) and peel (74-
78%), with slightly lower conversion in stem. MW-irradiation of alkali
slurry (5 min.) followed by Tween 20 supplemented saccharification was a
better strategy degrading cellulose and hemicellulose to very high
extent. Tween 20 supplementation was beneficial in enhancing the RS
release from the biomasses even when Cellic dosage was halved.
Ultrastructural studies indicated the disappearance of starch granules
from stem and peel samples after MW-irradiation and saccharification,
while fragmented cellulose fibers were visible in leaf samples. The
study showed that MW-assisted alkali pretreatment followed by
saccharification with Cellic in presence of Tween 20 was very effective
in releasing maximum sugars from these biomasses. 



10.22161/ijeab/2.2.10



http://ijeab.com/upload_document/issue_files/10%20IJEAB-FEB-2017-47-Microwave-Assisted%20Alkali%20Delignification%20Coupled%20with%20Non-Ionic%20Surfactant%20Effect.pdf



http://ijeab.com/submit-paper/

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