Comparative Studies on Cephalosporin C biosynthesis by Aspergillus and Acremonium Species

Comparative Studies on Cephalosporin C biosynthesis by Aspergillus and Acremonium Species



Zill-e-Huma Bilal, Adeela Yousaf, Iffat Siddique, Arusa Aftab



Three species of Aspergillus (A. niger, A. flavus, A.
nidulans) and two species of Acremonium (A. furcatum and A. kiliense)
were tested for their potential to biosynthesis of cephalosporin-C (CPC)
in batch mode of cultivation before and after mutagenesis. The effects
of different ingredients of the medium on CPC yield were thoroughly
investigated. Results manifested that sucrose 30 mg ml-1, DL methionine
3mg mL-1 and ammonium sulphate 7.5 mg mL-1 were most appropriate for
maximum harvest of antibiotic. Mutants were achieved by exposing fungal
cultures to 400 µg mL-1 of ethyl methane sulphonate (EMS) for 1 hour. 



10.22161/ijeab/1.4.43



http://ijeab.com/upload_document/issue_files/43%20IJEAB-NOV-2016-29-Comparative%20Studies%20on%20Cephalosporin%20C%20biosynthesis%20by%20Aspergillus%20and%20Acremonium%20Species.pdf



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