Evaluation of the euglycemic effect of oral administration of S. rebaudiana B. cultivated in Mexico in normoglycemic and induced-diabetic rats

Evaluation of the euglycemic effect of oral administration of S. rebaudiana B. cultivated in Mexico in normoglycemic and induced-diabetic rats



Aranda-Gonzalez Irma, Ortiz-Andrade Rolffy, Moguel-Ordonez Yolanda, Betancur-Ancona David



Stevia rebaudiana is a plant widely used as sweetener and food
supplement. There are some reports of the euglycemic effect of S.
rebaudiana extracts, which has been attributed mainly to stevioside for
the results obtained by other authors. However, studies with extracts
lack the precise quantification of glycosides and it is known that
several agricultural and environmental factors affect the steviol
glycosides content. The aim of this study was to evaluate the euglycemic
effect of oral administration of extracts of S. rebaudiana varieties
cultivated in Mexico in normoglycemic and induced-diabetic Wistar rats
and quantify their glycosides content. Aqueous leaves extracts of
Criolla and Morita II varieties of S. rebaudiana were used to quantify
glycosides by a validated HPLC method or lyophilized for in vivo
experiments. Hypoglycaemic effect was evaluated in normoglycemic fasting
rats, subjected to intragastric administration of Criolla or Morita II
(100 or 200 mg/kg) and glucose measured at time 0, 1, 3, 5 and 7 hours.
The antihyperglycemic effect was first evaluated with Streptozotocin /
Nicotinamide-induced diabetic rats following the methodology previously
described, and further evaluated with glucose load in normoglycemic
rats. Control groups were distilled water and glibenclamide (5 mg/kg).
Stevioside content was 2.08 ± 0.11 and 5.22 ± 0.23 (g/100 g of dry
leaves) in the Morita II and Criolla variety, respectively. Acute
administration of both varieties of S. rebaudiana had no euglycemic
effect in normoglycemic or induced-diabetic rats (p> 0.05) at any of
the doses tested. 



10.22161/ijeab/2.1.44



http://ijeab.com/upload_document/issue_files/44%20IJEAB-JAN-2017-62-Evaluation%20of%20the%20euglycemic%20effect%20of%20oral%20administration%20of%20S.%20rebaudiana%20B.%20cultivated.pdf



ijeab.com/submit-paper/

Comments

Post a Comment

Popular posts from this blog

The Ecological Impacts of Light Pollution at Night-time a Mechanistic Appraisal

The Ecological Impacts of Light Pollution at Night-time a Mechanistic Appraisal Abdullahi MG, Roslan U, Kamarudin M. K. A. Artificial light at night and its negative consequences are of great interest in the fields of Ecology, Human Geography Astronomy, and Human Health. Most of the studies to date concentrated on the influences of light pollution has on our ability to view the night sky, on biodiversity, and ecosystems. Though, in recent years, with the use of new technologies sensors, which provide accurate information for determining of night lights at the area, most consideration were given for estimating artificial light at night within the urban areas. In this study, urban night lights within the cities of Nigeria was evaluated by monitoring data from the ground measurements using Sky Quality Meter (SQM-LU-DL) devices. Besides, the SQM was used at the zenith (upward direction) for maximum evaluating of artificial light at the sites. Differences in night sky bri...
Read more

Studying the tendency of citizens to participate in the protection and development of land scape in Tehran

Studying the tendency of citizens to participate in the protection and development of land scape in Tehran Shadi Maleki, Abbas Mafi, Seyedeh Mahsa Hosseini Today, as an essential part and parcel of urban landscape that they play a major role in metabolism and create a lack of serious damage to urban life. This study performed to investigate the willingness of citizens to participate in activities to protect and develop landscapeof Tehran district 4. The research was descriptive and correlation method that is used for collecting information. The statistical population consisted of district 4 of Tehran. The sample size was determined 230 by using Cochrane method. A questionnaire was used to collect data. According to the research, 63.04 percent of citizens showed participation in a high level. Among the variables investigated how citizen participation in conservation and development of landscape, effective strategies to motivate citizens to participate,the reasons for la...
Read more

Land Use Land Cover Change Detection by Using Remote Sensing Data in Akaki River Basin

Land Use Land Cover Change Detection by Using Remote Sensing Data in Akaki River Basin Adimasu Woldesenbet Worako   Land use land cover change (LULCC) is the result of the long time process of natural and anthropogenic activities that has been practiced on the land. GIS and remote sensing are the best tools that support to generate the relevant land use/cover change in the basin. This study was conducted in the Akaki River basin to detect land use land cover changes within the 30 years period (1985-2015) by using landsat imagery data acquired from the GCF. Supervised maximum likelihood algorithm classification were deployed to classify land use/cover into four prominent land use groups and data’s were processed by using ERDAS imagine 2014 and ArcGIS10.1 software. In the basin dominant LULC was agricultural land use which accounts around 56.28% and the second largest is built-up area by 31.51% and the rest, forest(11.9%) and water body(0.31%) coverage were takes t...
Read more