Protease activity of extracellular enzyme produced by B. subtilis isolated from soil

Protease activity of extracellular enzyme produced by B. subtilis isolated from soil



Md. Gahangir alam, Md. Ekhlas uddin, Sezanur Rahman ,
Mohammad Reazul Karim, Md. Sharifull Islam, Tasnim Ahamad, Md Jakir
Hossain, Mohammad Shakil Mahmood, Mohammad Shah Alam, Pulak Maitra, Md.
Nazmuzzaman



Background: Proteases produced by enzymatic method are more environments
friendly than chemical process, and they have tremendous potential in
the leather industry and in other several industries. In this study
extracellular protease producing non pathogenic Bacillus subtilis was
isolated from soil sample and relationship between sporulation and
extracellular protease synthesis in large scale cultivation was studied.
The enzyme was further characterized, purified, and tested for
potential application.
Result: The molecular weight of the protease was found to be ~30 KDa.
Enzyme activity was checked on the presence of different metal ions and
effectors. The enzyme was slightly modulated by MG++ ion, and
significantly by Hg++ ion, while Zn++ ion slightly decrease the
proteolytic activity. Sulfahydryl reagents, DTT slightly and β-ME
significantly inhibit the enzyme. EDTA showed no effect on the enzyme
suggesting that the enzyme might not be metalloprotease. PMSF, a known
serine protease inhibitor was seen to totally inhibit the enzyme which
indicates that the enzyme is a serine protease. The optimum enzyme
activity was observed after 22 hours of incubation of B. subtilis at 37o
C.
Conclusions: Crude enzyme contains 285 units of enzyme which have direct
dehairing activity. The enzyme was also seen to be able to remove blood
and curry stain from clothes; making it a very promising candidate to
be used in a leather and detergent industry. Apart from protease the
bacterium was also seen to have lipase and collagenase activity. So, the
bacteria are potentially good candidate for industrial application. 



10.22161/ijeab/2.1.48



http://ijeab.com/upload_document/issue_files/48%20IJEAB-JAN-2017-25-Protease%20activity%20of%20extracellular%20enzyme%20produced%20by%20B.%20subtilis%20isolated%20from%20soil.pdf



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