In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction

In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction



Nair D.S., Reghunath B.R., Soni K.B., Alex S



An efficient protocol for rapid in vitro clonal propagation has been
established. Shoot proliferation was obtained from nodal explants in
Murashige and Skoog medium supplemented with 1.0 mg L-1BA and 0.1 mg L-1
IAA. The shoots were subsequently subcultured every four weeks. The
shoots were then rooted in vitro in MS medium supplemented with varying
concentrations of auxins. The most efficient rooting was observed in MS
supplemented with 1.5 mg L-1 IAA. The effective acclimatization (91.67
per cent) was obtained in sterilized sand. Slow growth was induced with
varying concentration of mannitol (5 g L-1 to 30 g L-1). Maximum slow
growth induction with 100 per cent regeneration was recorded in mannitol
(10 g L-1) supplemented medium. After 28 weeks in slow growth medium,
cultures when transferred to shoot proliferation medium gave 100 per
cent regeneration. The regenerants from slow growth induction were found
to be genetically stable. 



10.22161/ijeab/1.4.35



http://ijeab.com/upload_document/issue_files/35 IJEAB-DEC-2016-3-In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction.pdf



http://ijeab.com/submit-paper/

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