In Vitro Study on total Phenols, Flavonoids Content and DPPH Activity of Withania Species

In Vitro Study on total Phenols, Flavonoids Content and DPPH Activity of Withania Species



Nishtha Raj, Ayushi Agarwal, Neelam Chaturvedi



The escalating interest in appraisal of antioxidant
power of herbal plant as medicine, the current study was carried out to
explore the antioxidant potential of aqueous extracts of Withania
somnifera root and Withania coagulan fruit in-vitro. Antioxidant
activity; total phenol,total flavonoids and DPPH free radical scavenging
assay of Withania somnifera root and Withania coagulans fruit aqueous
extracts were determined by using reference standards gallic acid,
quercetin and ascorbic acid, respectively. The highest total phenols
content (mgGAE/g) and total flavonoids content (mgQE/g) was found to be
33.1±0.82 and 1.86±0.01 respectively in aqueous somnifera root extracts
as compared to coagulans fruit extract . The DPPH radical scavenging
activity of the both extracts was increased with the increasing
concentration and was observed high in aqueous extract insomniferaroot
(IC50= 54) than coagulans fruit (69μg/ml) aqueous extract.Thus,Withania
somnifera root has potent antioxidant activity and may serve as a good
pharmacotherapeutic agent which could be explored to provide affordable
medicines to masses. 



10.22161/ijeab/2.2.49



http://ijeab.com/upload_document/issue_files/49%20IJEAB-APR-2017-9-In%20Vitro%20Study%20on%20total%20Phenols%20and%20Flavonoids%20Content%20and%20DPPH%20Activity%20of%20Withania%20Spicies.pdf



ijeab.com/submit-paper/

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