Micro-propagation of Alstroemeria Hybrida Cv. Pluto

Micro-propagation of Alstroemeria Hybrida Cv. Pluto



Ambreena Din, Muneeb A. Wani, Sajid A. Malik, Shameen Iqbal, Imtiyaz T. Nazki



The experiment entitled micropropagation of Alstroemeria hybrida cv.
Pluto was conducted to standardize protocol for aseptic establishment,
callus induction, proliferation, and rooting from rhizome tips, rhizome
sections, shoot tips, shoot nodal segments and inflorescence buds.
Highest culture asepsis of 79.20 per cent at 2 weeks of culture and
68.08 per cent at 4 weeks of culture was recorded in rhizome tips
following sterilization treatment with Carbendazim 200 ppm for 30
minutes + HgCl2 (0.1 %) dip for 10 minutes and final treatment with
ethyl alcohol (70 %) for 1 minute. Rhizome tips and rhizome section
explants survived sterilant treatment better than other explants.
MS-liquid medium supplemented with BAP + IBA: 1.5 + 0.2 mg l-l proved
best for culture establishment (89.42 %) in case of rhizome tips and
(56.13 %) in case of rhizome sections. MS-solid medium with plant growth
regulator combinations BAP + IBA: 1.0 + 0.2 mg l-1 fortified with
activated charcoal resulted in an establishment of (78.25 %) in rhizome
tips and (40.24 %) in case of rhizome sections. Callus induction was
highest in MS-solid medium fortified with BAP + NAA: 0.5 + 4.5 mg l-l.
Rhizome tips cultured on MS-medium BAP + IBA + GA3 + Activated charcoal:
2.0 + 0.4 + 0.5 + 1000 mg l-l gave highest proliferation (88.85 %)
along with highest number of erect shoots (5.75) , number of new rhizome
buds ( 3.75), rhizome fresh weight/shoot complex (6.05), and
multiplication index (2.76). Highest Rooting (54.81 %) along with lowest
days to appearance of root (10.87), highest number of roots (3.12) and
highest root length (16.42 mm) was recorded in MS-liquid medium
fortified with NAA 1.5 mg l-1.
Abbreviations used— AC; Activated charcoal, BAP; 6-Benzyl amino purine,
BA; 6-Benzyladenine, 2, 4-D; 2, 4dichloro-phenoxyacetic acid,GA3;
Gibberelic acid, IAA; Indole-3-acetic acid, IBA; Indole-3-butyric acid,
MS; Murashige and Skoog’s (1962) medium, NAA; Naphthalene acetic acid
and µm; Micro molar. 



10.22161/ijeab/2.2.13



http://ijeab.com/upload_document/issue_files/13%20IJEAB-MAR-2017-11-Micro-propagation%20of%20Alstroemeria%20Hybrida%20Cv.%20Pluto.pdf



ijeab.com/submit-paper/

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